34.LEVINTHAL’S AGAR Solution A: Neopeptone BD216881 5,0 g NaCl 2,5 g Bacto-Beef Extract BD211520 5,0 g Agar bacteriological (Agar No. 1) Oxoid LP0011 10,0 g Distilled water 500,0 ml Adjust pH to 7,6; sterilize at 121°C for 15 minutes and store in water bath at 55°C. Solution B: Brain Heart Infusion Oxoid CM0225 29,2 g Distilled water 790,0 ml Adjust to 7,6 pH; boil and add 80 ml of defibrinated sheep blood. While hot, filter through filter paper, sterilize by filtration and cool to 55°C. Pool equal part of solutions A and B, distribute into Petri dishes. |
|||
|
|||