65.GC MEDIUM Solution A GC agar base (BD 212171, Oxoid CM0367) 36,0g Agar (if needed) 5,0g Distilled water 500,ml Solution B Dried bovine hemoglobin (BD 212392, Oxoid LP0053) 10,0g Distilled water 500,0ml Solution C IsoVitalex enrichment (BD 211876) or Vitox (Oxoid SR0090) 10,0ml Autoclave solutions A and B separately at 121°C for 15 minutes. Cool to 50°C. Aseptically add solution B to solution A. Mix very well. Rehydrate IsoVitalex (or Vitox) according to package instructions and add aseptically to medium. Mix well. |
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