Cent Eur J Public Health 2003, 11(Supplement)
Multipurpose serological survey - its philosophy and objectives in the Czech Republic
Kříž B.
Cent Eur J Public Health 2003, 11(Supplement):S4-S6 
The 2001 serological survey in the Czech Republic - Diphtheria
Kříž B.
Cent Eur J Public Health 2003, 11(Supplement):S7-S12
Diphtheria morbidity in the Czech part of former Czechoslovakia showed a continuous downward trend between 1946 and 1974. Afterwards, sporadic cases of diphtheria were reported in some years.Compulsory vaccination against diphtheria was started in 1946 with a monovaccine, later replaced by bivaccine DiTe. Since 1958, newborns have been vaccinated with DiTePe vaccine. As many as 98 % to 100 % of the population of age groups under 50 years likely to have been vaccinated have antibody levels >0.01 IU/ml. About 83 % to 88 % of the older age groups who represent a naturally immunized population have antibodies as well. This immune status excludes...
The 2001 serological survey in the Czech Republic - Tetanus
Janout V., Matoušková I., Čížek L.
Cent Eur J Public Health 2003, 11(Supplement):S13-S16
In 2001, a serological survey of antibody against tetanus was carried out in the Czech Republic among 499 individuals aged between 40 and 64 years. It revealed that 2.8 % of them had antibody titres below protective levels and 43.3 % and 53.9 % respectively had medium and high antibody titres, i.e. protective levels of antibodies. These results show a good immunity level against tetanus among the Czech population aged between 40 and 64 years, likely to be ascribed to the good immunization coverage achieved. The individuals with antibody titres below protective levels need revaccination.
The 2001 serological survey in the Czech Republic - Pertussis
Maixnerová M.
Cent Eur J Public Health 2003, 11(Supplement):S17-S22
The results of the 2001 serological survey show that the immunity status of 2-year old children has improved (89.7% seropositive) after the modification of the vaccination schedule while the immunity status for one-year-olds remains poor (57.6% seropositive). The highest prevalence of seropositivity was seen in the age categories from 12 to 44 years (90-97%). The increasing values of seropositivity and relatively higher titers of antibodies (in particular against type 1,3 of B. pertussis) in older children and adolescents likely reflect an ongoing infection in this population.
The 2001 serological survey in the Czech Republic - Parapertussis
Maixnerová M.
Cent Eur J Public Health 2003, 11(Supplement):S23-S24
The results of the 2001 serological survey of antibodies against parapertussis confirmed a relatively high immunity rate of the Czech population. Prevalence of positive titers of antibodies can reach 60% in children 10 years old and ranges from 40 to 60% in the adult population. A rapid increase of immunity rate of the population suggests a high circulation of B. parapertussis in the population.
The 2001 serological survey in the Czech Republic - Hib invasive disease Haemophilus influenzae b
Lebedová V., Křížová P.
Cent Eur J Public Health 2003, 11(Supplement):S25-S30
Dynamics in total antibody levels against Haemophilus influenzae b (Hib) among the Czech population were monitored for the first time within the 2001 serological survey. In total, 2,479 non-vaccinee sera and 65 vaccinee sera from the population of age groups 1-39 years were tested. The total anti-Hib antibody levels determined by means of ELISA were higher than 0.15 µg/ml for all of the sera tested. Levels of 1 µg/ml or higher were recorded for 97% and 99% of vaccinee and non-vaccinee sera, respectively.The non-vaccinees showed the highest rates of levels below 1 µg/ml in the age groups of 1-, 2-, 6- and 12-year-olds (4%, 7%,...
The 2001 serological survey in the Czech Republic - Poliomyelitis
Matyášová I., Rainetová P., Částková J.
Cent Eur J Public Health 2003, 11(Supplement):S31-S35
Regular vaccination against poliomyelitis was started in 1960 with oral polio vaccine (OPV). Since 1992 a trivalent OPV has been administered in five doses within a nationwide vaccination campaign. The immunization coverage varies between 96.8 % and 98.2 % after 4 OPV doses, reaching 98.0 % to 98.9 % after the fifth dose. No case of indigenous poliomyelitis has been reported in the Czech Republic since the second half of 1960. In 2001, 3,230 sera were tested for the presence of antibodies against poliovirus of types 1, 2 and 3 using a virus neutralization microassay. The prevalence rates of antibodies vary between 96.0 % and 100 % for types 1 and 2...
The 2001 serological survey in the Czech Republic - Measles
Mrázová M., Švandová E., Šmelhausová M., Šestáková Z., Beneš Č.
Cent Eur J Public Health 2003, 11(Supplement):S36-S41
In total, 3,013 sera from a representative population sample of the Czech Republic aged from 1 to 64 years were tested according to the requirements of the ESEN 2 project using a commercial kit Measles IgG (II) EIA manufactured by Denka Seiken, Japan. The highest seropositivity rate and GMT values were found in the population group aged over 35 years coming into natural contact with the causative agent. Among the vaccinee population, the best outcomes were achieved in 2-9-year-olds (seropositivity rate: 94-100 %, GMT: 2500-4000 EIA) and 10-14-year-olds (seropositivity rate: 93-97.1 %, GMT almost 2000 EIA). The lowest seropositivity rate was found in...
The 2001 serological survey in the Czech Republic - Rubella
Mrázová M., Šmelhausová M., Šestáková Z., Švandová E., Beneš Č.
Cent Eur J Public Health 2003, 11(Supplement):S42-S49
In total, 3,009 sera from a representative population sample of the Czech Republic, 1508 females and 1,501 males, were tested by EIA using a commercial kit ETI-RUBEK-G Plus manufactured by Dia Sorin, Italy. The vaccination strategy was as follows: vaccination of 12-year-old girls since 1982 and all 2-yearolds vaccinated since 1986. Currently, all women under 31 years of age and all men under 17 years of age have been vaccinated. The results of the serological survey confirmed lower seropositivity rates in young men compared to vaccinated women of the same age as expected. An epidemic outbreak of rubeola among young men is another evidence of that....
The 2001 serological survey in the Czech Republic - Mumps
Mrázová M., Šmelhausová M., Šestáková Z., Švandová E., Beneš Č.
Cent Eur J Public Health 2003, 11(Supplement):S50-S53
In total, 3,010 sera from a representative population sample of the Czech Republic aged from 1 to 64 years were tested according to the requirements of the ESEN 2 project using a commercial kit Mumps IgG (II) EIA manufactured by Denka Seiken, Japan. To date, the regular programme of vaccination against mumps has covered the population of children under 15 years of age. The vaccination coverage achieved (97-100 %) does not correspond to the antibody prevalence rates of 70.2 to 86.4 % as found. After implementation of regular vaccination in 1987, the morbidity rates have fallen dramatically. Nevertheless, the collective immunity achieved is not sufficient...
The 2001 serological survey in the Czech Republic - Viral hepatitis
Němeček V., Částková J., Fritz P., Linhartová A., Švandová E., Šrámová H., Kříž B.
Cent Eur J Public Health 2003, 11(Supplement):S54-S61
Within serological surveys 2001, prevalence of markers of hepatitis viruses A (anti-HAV), B (anti-HBc, HBsAg, anti-HBs) and for the first time also C (anti-HCV) was investigated. Sera were collected in 2001and tested by respective kits AxSYM, Abbott.HAV: 2,623 sera were tested for the presence of anti-HAV antibodies. Comparison with serological surveys of 1984 and 1996 revealed again shifts of the age prevalence curve for anti-HAV antibodies towards higher age groups corresponding to time intervals between epidemiological surveys. High prevalence rates of anti-HAV antibodies (more than 20 %) were only found for the population age groups who lived...
The 2001 serological survey in the Czech Republic - Invasive meningococcal disease
Křížová P.
Cent Eur J Public Health 2003, 11(Supplement):S62-S67
In 2001, 730 sera from the population of 14 areas of the Czech Republic aged between 1 and 64 years were tested by a microplate assay for the presence of bactericidal antibodies against N. meningitidis A:4,21:P1.10, N. meningitidis B:2a:P1.2,P1.5 and N. meningitidis C:2a:P1.2,P1.5. The Czech population showed high immunity against N. meningitidis A:4,21:P1.10, but low immunity against N. meningitidis B:2a:P1.2,P1.5 and N. meningitidis C:2a:P1.2,P1.5. The current epidemiological situation requires neither vaccination against meningococcus A nor inclusion of conjugated vaccine against meningococcus C into routine vaccination. Nevertheless, the invasive...